Angiogenesis

Xenograft-CAM assay control

Xenograft-CAM assay with treatment

The Southern Research Angiogenesis Program provides an integrated series of in vitro and in vivo assays and analyses to evaluate compounds and biological agents affecting the angiogenesis process.

In vitro assays

• Endothelial cell proliferation, migration/invasion, and tube-formation
• Human endothelial progenitor cell (EPC) proliferation, migration/invasion, and tube-formation
• Three dimensional (3D) angiogenesis assays under influence of other cell types with co-culture systems
• 3D co-culture assay system for cell-cell interactions in tumor microenvironment
• Angiogenesis assays for high throughput screening (HTS)

In vivo assays and analyses

• Chick chorioallantoic membrane (CAM) assay with quantitative analysis of new blood vessel formation
• Tumor angiogenesis xenograft-CAM assay where human tumor cells are implanted on the CAM, and new blood vessel formation and tumor growth are quantitatively analyzed
• Matrigel plug assay in mice with quantitative analysis of microvessel density (MVD)
• Analysis of angiogenic effect for xenograft mouse study, including tumor MVD and levels of angiogenic growth factors in blood serum and tumor samples
• A new xenograft mouse model with regulated tumor angiogenesis and inducible metastatic phenotype, used in preclinical efficacy studies to test potential anticancer drugs and to evaluate new treatment modifications

The Angiogenesis Program at Southern Research has evaluated several thousand compounds as anti- or pro-angiogenic agents in recent years for a variety of clients. We are flexible in assay and experiment design based on the specific objectives of each study.