A meeting of Southern Research’s Institutional Biosafety Committee convened on Wednesday, 01 Oct 2025, in Birmingham, Alabama 35205.
The following participants were present via Teams:
Birmingham Site
Participant, Voting Status
Kayton Cherry, Voting
Amanda Haley, Guest
Christina Humphries, Ex-Officio
Mark Jackson, Voting
Steven Orr
Carson Sakamoto
James Toomey
Anwar Ullah, Guest
1. 2. Voting
Alternate
Voting
Review and Approval of Meeting Agenda
The Chairperson called the meeting to order. The Chairperson asked the committee to
review the agenda. A motion was made to approve the agenda. A second was made, and
by voice vote the agenda was approved.
Review and Approval of BPRs and Amendments
R25-09-017NS
Title: Production and purification of recombinant protein from DNA plasmid in bacteria,
insect, and mammalian cell
RS: Anwar Ullah
The BPRG was reviewed, comments and contingencies were received.
Contingency 1: Section 5.13a is incomplete.
Response:
BSL-2 box checked.
Contingency 2: For section 5.14a, should it be 10% bleach?
Response:
Changed to “The bacteria will be treated with a 1:9 bleach-to-water solution (Pure Bright
bleach solution (sodium hypochlorite (6%)) solution for 1 hour before being discarded into the
sink.”
Contingency 3: Section 6.4d is incomplete.
Response:
Added “1 mL of pure protein (10 mg/mL)”.Institutional Biosafety Committee Minutes, Page 2 of 2
Contingency 4: For section 6.6a, what is the animal cells or tissues species, strains, and
quantity for all?
Response:
Checked “Bacteria” box and added “(E. coli BL21 DE3 strain)”.
Contingency 5: 5.11 (e, f): If there are no in vivo activities, uncheck the “NO” box.
Response:
Boxes unchecked.
Contingency 6: 8.4 (e, f): uncheck the “NO” box.
Response:
Boxes unchecked.
Contingency 7: Section 1.6: (b) Have the facilities to be used for this project been
inspected to ensure that they are appropriate for the cited Biosafety Levels? If NO, then you
MUST contact the Biological Safety Officer regarding inspection prior to initiation of the
work. – Has this been completed?
Response:
It has not been completed since we have just moved the BSC and the shaker incubator to
NRB. Steve will make sure the inspection is completed.
A motion was made to approve, a second was made, and by voice vote the BPR was
approved.
3. Next Meeting
The next Institutional Biosafety Committee meeting is scheduled for Thursday, 09 Oct 2025
at 11:15 am in Birmingham.
4. Adjourn