Students will be introduced to a family interested in finding out whether their child has the disease, Cystic Fibrosis (CF). Students will use gel electrophoresis to detect a mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene and determine if the individuals do or do not have the disease or if they are a carrier for CF. Following the diagnosis, students will learn about the Central Dogma of biology and its role in individuals who have Cystic Fibrosis. Through a hands-on activity, students will transcribe, translate, and synthesize the CFTR protein – a critical chloride protein channel. They will then use the 3D protein models to discuss how a mutation in the CFTR gene can result in improper protein function, which results in the key symptoms seen in those with CF.
*Full experience is 90 minutes but can be split into two 45-minute sessions across two days for those on 50-minute period schedules.